7FG8

H/D exchanged Hen egg-white lysozyme denatured in acidic conditions and refolded in solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2018-02-13
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	79.182, 79.182, 37.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.990 - 2.000
Rwork	0.233
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k8g
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.027	0.080
Number of reflections	8580	808
<I/σ(I)>	67.6
Completeness [%]	99.6
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NACL, SODIUM ACETATE-D3, ACETIC ACID-D4
1	VAPOR DIFFUSION, SITTING DROP		293	NACL, SODIUM ACETATE-D3, ACETIC ACID-D4