Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.732, 111.968, 110.873 |
| Unit cell angles | 90.00, 110.87, 90.00 |
Refinement procedure
| Resolution | 38.050 - 3.380 |
| R-factor | 0.2925 |
| Rwork | 0.290 |
| R-free | 0.33710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2omf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.440 |
| High resolution limit [Å] | 3.380 | 9.160 | 3.380 |
| Rmerge | 0.140 | 0.119 | 0.441 |
| Rmeas | 0.152 | 0.133 | 0.473 |
| Rpim | 0.057 | 0.057 | 0.172 |
| Total number of observations | 184541 | ||
| Number of reflections | 24876 | 1182 | 1250 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.5 | 91.2 | 100 |
| Redundancy | 7.4 | 6.3 | 7.6 |
| CC(1/2) | 0.947 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 mM sodium cacodylate (pH 8.0) buffer containing 45% PEG 200, 0.12 M MgCl2 |
