7FF4

The crystal structure of Clostridium cellulolyticum LacI family transcriptional regulator Ccel_1438

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	H 3
Unit cell lengths	194.518, 194.518, 118.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.629 - 2.190
R-factor	0.1793
Rwork	0.178
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzr
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.071	0.760
Number of reflections	85663	8185
<I/σ(I)>	18.6
Completeness [%]	99.5
Redundancy	9.6
CC(1/2)	0.998	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.1 M Bis Tris Propane pH 6.5, 0.2 M Trisodium citrate dihydrate, 20% w/v PEG 3350, 12% v/v Ethylene glycol