7FEO
Crystal structure of AtMBD5 MBD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-15 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 72.852, 72.852, 120.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.450 - 2.200 |
| R-factor | 0.2365 |
| Rwork | 0.233 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c1t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.450 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 8605 | 854 |
| <I/σ(I)> | 29.76 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14.3 | |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 2M ammonium sulphate, 0.2M Sodium Chloride, 0.1M cacodylate pH5.5 |
