7FDZ
Levansucrase from Brenneria sp. EniD 312 with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.125, 98.125, 215.534 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.980 - 1.350 |
| R-factor | 0.1301 |
| Rwork | 0.129 |
| R-free | 0.14980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d47 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.545 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.980 | 1.200 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Number of reflections | 161790 | 578 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 80.7 | 5.9 |
| Redundancy | 38.1 | |
| CC(1/2) | 1.000 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium sulfate, PEG 400 |
