7FDY

Structure of OmpF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-07-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97919
Spacegroup name	C 1 2 1
Unit cell lengths	128.600, 140.390, 110.860
Unit cell angles	90.00, 120.01, 90.00

Refinement procedure

Resolution	29.128 - 3.100
R-factor	0.2706
Rwork	0.269
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2omf
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.128	29.128	3.280
High resolution limit [Å]	3.100	8.900	3.100
Rmerge	0.109	0.029	0.777
Rmeas	0.127	0.034	0.908
Total number of observations	226132
Number of reflections	60349	2445	9678
<I/σ(I)>	10.33	35.31	1.55
Completeness [%]	98.3	93.5	98.1
Redundancy	3.747	3.333	3.72
CC(1/2)	0.997	0.998	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.1 mM sodium cacodylate (pH 8.0), 43% PEG 200, 0.12 M MgCl2