7FDW
Crystal structure of pepsin cleaved lactoferrin C-lobe at 2.28 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96546 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.781, 81.703, 111.694 |
| Unit cell angles | 90.00, 129.86, 90.00 |
Refinement procedure
| Resolution | 76.700 - 2.277 |
| Rwork | 0.213 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1blf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.607 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.310 |
| High resolution limit [Å] | 2.277 | 2.277 |
| Rmerge | 0.030 | 0.440 |
| Number of reflections | 48821 | 3392 |
| <I/σ(I)> | 17.6 | 2.9 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 3 | 3.2 |
| CC(1/2) | 0.970 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Magnesium acetate, 20% PEG 3350 |
