7FDN
Crystal structure of transcription factor WER in complex with EGL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.452, 70.452, 88.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.540 - 1.900 |
| R-factor | 0.1873 |
| Rwork | 0.185 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rru |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.059 | 0.030 | 0.953 |
| Rmeas | 0.063 | 0.032 | 1.018 |
| Rpim | 0.020 | 0.010 | 0.350 |
| Total number of observations | 379714 | ||
| Number of reflections | 38966 | 3907 | 3898 |
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.8 | 99.7 | 99.2 |
| Redundancy | 9.7 | 9.7 | 7.7 |
| CC(1/2) | 0.999 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM CAPS pH 10.5, 200 mM lithium sulfate, and 2 M ammonium sulfate |
