7FDN

Crystal structure of transcription factor WER in complex with EGL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97930
Spacegroup name	P 32
Unit cell lengths	70.452, 70.452, 88.556
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.540 - 1.900
R-factor	0.1873
Rwork	0.185
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rru
RMSD bond length	0.012
RMSD bond angle	1.689
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.059	0.030	0.953
Rmeas	0.063	0.032	1.018
Rpim	0.020	0.010	0.350
Total number of observations	379714
Number of reflections	38966	3907	3898
<I/σ(I)>	11.5
Completeness [%]	99.8	99.7	99.2
Redundancy	9.7	9.7	7.7
CC(1/2)		0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM CAPS pH 10.5, 200 mM lithium sulfate, and 2 M ammonium sulfate