7FDM

Crystal structure of transcription factor MYB29 in complex with MYC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97930
Spacegroup name	P 32
Unit cell lengths	85.247, 85.247, 57.017
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.900 - 2.500
R-factor	0.2355
Rwork	0.232
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rru
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.041	0.026	0.421
Rmeas	0.043	0.028	0.491
Rpim	0.015	0.009	0.244
Total number of observations	98697
Number of reflections	15757	1617	1489
<I/σ(I)>	11.6
Completeness [%]	97.5	99.8	91.3
Redundancy	6.3	9.7	3.3
CC(1/2)		0.999	0.114

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 7.0, 20% (w/v) PEG 6000, 0.2 M Sodium chloride