7FDL

Crystal structure of transcription factor WER in complex with EGL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97930
Spacegroup name	C 2 2 21
Unit cell lengths	75.913, 193.392, 224.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.907 - 2.897
R-factor	0.2565
Rwork	0.254
R-free	0.29850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rru
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (7.0.076)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.000
High resolution limit [Å]	2.897	6.230	2.900
Rmerge	0.148	0.053	0.708
Rmeas	0.157	0.056	0.770
Rpim	0.052	0.017	0.292
Number of reflections	37043	3912	3585
<I/σ(I)>	4.7
Completeness [%]	99.7	99.9	98.4
Redundancy	8.3	10.4	6.1
CC(1/2)		0.999	0.114

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG3350, 200 mM magnesium formate