7FCU
X-ray structure of D2O-solvent lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.140, 79.140, 37.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.390 - 1.420 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iee |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.052 |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | XDS (Mar 15, 2019) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.570 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.042 | 0.642 |
| Rmeas | 0.044 | 0.665 |
| Rpim | 0.015 | 0.171 |
| Number of reflections | 23269 | 1130 |
| <I/σ(I)> | 36.6 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.8 | 15 |
| CC(1/2) | 1.000 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.5 | 293 | 15-25mg/mL HEWL, 50mM sodium acetate (pH4.5), 0.1g/mL NiCl2 |
