7FC6

Crystal structure of SARS-CoV RBD and horse ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	57.963, 126.314, 171.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.946 - 2.655
R-factor	0.1874
Rwork	0.185
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ajf
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.950	2.750
High resolution limit [Å]	2.655	2.655
Number of reflections	37045	3573
<I/σ(I)>	16.7
Completeness [%]	99.3
Redundancy	8.9
CC(1/2)	0.994	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.03M Citric acid/0.07M BIS-TRIS propane pH7.6, 20% w/v polyethylene glycol 3350