7FBY

Crystal Structure of PH0140 from Pyrococcus horikosii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-11-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	I 4 2 2
Unit cell lengths	102.790, 102.790, 69.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.711 - 2.001
Rwork	0.173
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cyy
RMSD bond length	0.015
RMSD bond angle	1.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.087	0.660
Number of reflections	12806	1258
<I/σ(I)>	32.54
Completeness [%]	99.9
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M HEPES (PH 7.5), 4.3 M sodium chloride