Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9643 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.384, 45.384, 250.522 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.290 - 1.900 |
| R-factor | 0.2751 |
| Rwork | 0.273 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.290 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.080 | 0.750 |
| Number of reflections | 23722 | 3113 |
| <I/σ(I)> | 30.94 | 3.46 |
| Completeness [%] | 95.6 | 80.1 |
| Redundancy | 27.82 | 11.66 |
| CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 200 mM MgCl2, 30 % (w/v) PEG 4000, 100 mM Tris-Cl pH 8.5 |
