7FAX

Complex structure of TbLeo1 and LW domain from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	I 4
Unit cell lengths	79.919, 79.919, 38.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.969 - 1.800
R-factor	0.2207
Rwork	0.215
R-free	0.23840
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.077	0.538
Number of reflections	22291	1633
<I/σ(I)>	75.7	3.4
Completeness [%]	100.0	100
Redundancy	12.8
CC(1/2)	0.990	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	3.5M NaCOOH, pH 7.0