7FAU

Structure Determination of the NB1B11-RBD Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	97.15
Detector technology	PIXEL
Collection date	2021-01-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 21 2 21
Unit cell lengths	62.512, 88.835, 190.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.530 - 2.080
R-factor	0.1999
Rwork	0.198
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ch5
RMSD bond length	0.006
RMSD bond angle	0.871
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	64558	44904
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	9.9
CC(1/2)	0.999	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	16% PEG3350 and 0.1 M Zinc acetate, dehydrate