7FAT

Structure Determination of the RBD-NB1A7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	97.15
Detector technology	PIXEL
Collection date	2021-01-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	C 2 2 2
Unit cell lengths	110.663, 183.795, 42.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 1.990
R-factor	0.1963
Rwork	0.193
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ch5
RMSD bond length	0.010
RMSD bond angle	1.092
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.720	2.070
High resolution limit [Å]	1.990	2.000
Number of reflections	27746	30986
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.960	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	8% PEG6000, 0.1 M citric acid pH 3.5 and 2% ethylene glycol