7FAG
Room temperature structure of elastase with high-strength agarose hydrogel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.209, 58.116, 75.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.910 - 1.300 |
| R-factor | 0.11701 |
| Rwork | 0.115 |
| R-free | 0.15288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5avd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rpim | 0.044 | 0.314 |
| Number of reflections | 54930 | 2663 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 98.2 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M Na acetate (pH8.5), 0.03M ammonium sulfate |
