7F9K
Crystal structure of the variable region of Plasmodium RIFIN #6(PF3D7_1400600)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-25 |
| Detector | SDMS |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.127, 24.496, 36.642 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 36.390 - 2.180 |
| R-factor | 0.2089 |
| Rwork | 0.206 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f9l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.180 | 2.200 |
| Number of reflections | 7466 | 7466 |
| <I/σ(I)> | 37.98 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.998 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M PCTP buffer, 25% w/v polyethylene glycol 1,500, pH 7.0 |
