7F9C
Homo sapiens Prolyl-tRNA Synthetase (HsPRS) in Complex with L-proline and compound L96
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.590, 91.660, 83.380 |
| Unit cell angles | 90.00, 110.28, 90.00 |
Refinement procedure
| Resolution | 62.293 - 2.196 |
| R-factor | 0.1911 |
| Rwork | 0.188 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vad |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.293 | 2.330 |
| High resolution limit [Å] | 2.196 | 2.196 |
| Rmerge | 0.131 | 0.833 |
| Rmeas | 0.142 | 0.909 |
| Number of reflections | 99589 | 8174 |
| <I/σ(I)> | 7.25 | 0.48 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 1.5 M CaCl2, 205 PEG 3350 |
