7F8E

Crystal structure of YggS from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	37.929, 146.375, 74.128
Unit cell angles	90.00, 93.36, 90.00

Refinement procedure

Resolution	36.590 - 2.080
R-factor	0.1742
Rwork	0.172
R-free	0.21910
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.873
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.590	2.154
High resolution limit [Å]	2.080	2.080
Number of reflections	47064	1175
<I/σ(I)>	27.13	4.333
Completeness [%]	97.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	295	0.1M sodium acetate trihydrate pH4.8, 0.19M Ammonium Sulfate , 21% (w/v) PEG 4000