7F8C
Crystal structure of rRNA methyltransferase Erm38 in complex with sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 78.018, 78.018, 101.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.470 - 2.248 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f8a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.470 | 48.470 | 2.320 |
| High resolution limit [Å] | 2.248 | 8.990 | 2.250 |
| Rmerge | 0.079 | 0.023 | 0.800 |
| Rmeas | 0.080 | 0.023 | 0.818 |
| Rpim | 0.016 | 0.005 | 0.167 |
| Total number of observations | 6478 | 31343 | |
| Number of reflections | 15503 | 308 | 1344 |
| <I/σ(I)> | 31.3 | 95.4 | 4.4 |
| Completeness [%] | 99.8 | 99.6 | 97.5 |
| Redundancy | 25.9 | 21 | 23.3 |
| CC(1/2) | 1.000 | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M succinic acid pH 7, 5% glycerol |
