7F72

Rv3094c in complex with FAD and ETH.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	289.15
Detector technology	CCD
Collection date	2019-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	134.100, 134.100, 109.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.379 - 1.640
R-factor	0.1865
Rwork	0.185
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kcf
Data scaling software	Aimless
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.380	1.699
High resolution limit [Å]	1.640	1.640
Number of reflections	60945	6039
<I/σ(I)>	25.8
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.996	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		289.15	0.2M sodium nitrate, 0.1M Bis Tris propane pH=8.5, 20% w/v PEG3350.