7F71
Crystal structure of the Mycobacterium tuberculosis L,D-transpeptidase-2 (LdtMt2) with peptidoglycan sugar moiety and glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.906, 93.981, 75.539 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.580 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e1g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.730 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.060 | 0.572 |
| Rmeas | 0.067 | 0.653 |
| Rpim | 0.030 | 0.306 |
| Number of reflections | 173244 | 5750 |
| <I/σ(I)> | 22 | 1.8 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 4.3 | 4.1 |
| CC(1/2) | 0.900 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% 5000MME, 200mM ammonium sulfate |
