7F70

Crystal structure of Rv3094c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	289.15
Detector technology	CCD
Collection date	2019-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	134.322, 134.322, 110.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.160 - 2.140
R-factor	0.1963
Rwork	0.194
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kcf
Data scaling software	HKL-3000
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.160	2.217
High resolution limit [Å]	2.140	2.140
Number of reflections	49234	2750
<I/σ(I)>	7.53
Completeness [%]	93.0
Redundancy	2
CC(1/2)	0.708	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		289.15	0.2M sodium nitrate, 0.1M Bis Tris propane pH=8.5, 20% w/v PEG3350