7F6Z
Complex Structure of antibody BD-503 and RBD-501Y.V2 of COVID-19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-06 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.920, 146.837, 145.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.994 - 3.000 |
| R-factor | 0.2115 |
| Rwork | 0.207 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f6y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.347 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.994 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.200 | 1.000 |
| Rmeas | 0.220 | |
| Rpim | 0.090 | 0.430 |
| Number of reflections | 36180 | 1777 |
| <I/σ(I)> | 8.33 | 1.5 |
| Completeness [%] | 99.5 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Ammonium sulfate, 0.1M Tris, pH 8.5, 12% (w/v) PEG 8000 |
