7F5W

Conserved and divergent strigolactone signaling in Saccharum spontaneum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	48.810, 88.295, 118.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.806 - 1.654
R-factor	0.1927
Rwork	0.191
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.810	1.740
High resolution limit [Å]	1.650	1.650
Rmerge		0.744
Rmeas	0.115
Number of reflections	110054	58049
<I/σ(I)>	12.1
Completeness [%]	93.6
Redundancy	6.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.01 M Magnesium chloride hexahydrate, 0.05 M TRIS hydrochloride pH 7.5, 5% v/v 2-Propanol