7F3N
Structure of PopP2 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.360, 89.360, 79.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.489 - 2.352 |
| R-factor | 0.196863902294 |
| Rwork | 0.193 |
| R-free | 0.23136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w3t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 15654 | 1503 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.2 | |
| CC(1/2) | 0.984 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 Hepeps, pH7.5, 50 mM MgCl2, 30% PEG55OMME |
