7F3J

Crystal structure of human YBX2 CSD in complex with m5C RNA in space group P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	42.616, 46.364, 57.704
Unit cell angles	69.26, 85.60, 90.02

Refinement procedure

Resolution	24.740 - 1.950
R-factor	0.1903
Rwork	0.188
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a6j
RMSD bond length	0.007
RMSD bond angle	1.102
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.071	0.276
Number of reflections	29797	1385
<I/σ(I)>	14.2
Completeness [%]	97.1
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	30% (w/v) PEG 4000, 0.1 M Tris-HCl, pH 8.5, 0.2 M Lithium Sulfate