7F3I
Crystal structure of human YBX2 CSD in complex with m5C RNA in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.532, 59.348, 118.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.670 - 2.250 |
R-factor | 0.1909 |
Rwork | 0.185 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a6j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.124 | 0.477 |
Number of reflections | 15180 | 757 |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.9 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M Imidazole, 20% (w/v) PEG 8000, 3% (v/v) MPD |