7F39

The structure of flavin transferase FmnB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	65.913, 66.768, 87.493
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.592 - 1.888
Rwork	0.191
R-free	0.22420
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.869
Data reduction software	xia2 (0.7.7)
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.592	1.958
High resolution limit [Å]	1.888	1.890
Rmerge	0.010
Number of reflections	31577	3102
<I/σ(I)>	418.2
Completeness [%]	99.7
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.2M sodium formate, 20% w/v Polyethylene glycol 3350