7F2V

Urate oxidase from Thermobispora bispora in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-22
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	C 1 2 1
Unit cell lengths	137.466, 78.051, 65.720
Unit cell angles	90.00, 116.35, 90.00

Refinement procedure

Resolution	24.180 - 1.600
R-factor	0.1679
Rwork	0.167
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oe8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.180	1.650
High resolution limit [Å]	1.600	1.600
Number of reflections	81042	7321
<I/σ(I)>	5.1
Completeness [%]	99.9
Redundancy	4.2
CC(1/2)	0.935	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Bis-Tris, lithium sulfate monohydrate, PEG3350