7F2S
Crystal structure of anti S-gatifloxacin antibody Fab fragment apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-01-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.058, 76.058, 378.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.580 - 2.620 |
R-factor | 0.2485 |
Rwork | 0.247 |
R-free | 0.27760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1egj |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.360 | 2.740 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.095 | |
Rmeas | 0.098 | |
Rpim | 0.021 | |
Number of reflections | 20813 | 2452 |
<I/σ(I)> | 33.6 | 2.3 |
Completeness [%] | 100.0 | |
Redundancy | 36.5 | |
CC(1/2) | 1.000 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.8 M sodium acetate pH 4.6, 1.6 M ammonium sulfate, 24% glycerol |