Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2021-04-29 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.388, 57.007, 93.800 |
| Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
| Resolution | 27.690 - 2.350 |
| Rwork | 0.211 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyg |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.064 | 0.286 |
| Number of reflections | 22807 | 2047 |
| <I/σ(I)> | 17.22 | 3 |
| Completeness [%] | 97.7 | 89.9 |
| Redundancy | 3.4 | 2.9 |
| CC(1/2) | 0.984 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Magnesium acetate tetrahydrate, 20% PEG 3350, pH6.5 |
