7F2I

Arabidopsis thaliana protease-associated domain of vacuolar-sorting receptor 1 in complex with cruciferin 1 C-terminal pentapeptide RVAAA (pH6.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2018-11-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	P 1 21 1
Unit cell lengths	32.689, 60.724, 40.444
Unit cell angles	90.00, 111.66, 90.00

Refinement procedure

Resolution	30.380 - 2.350
R-factor	0.1888
Rwork	0.185
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tjx
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.590	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.069	0.208
Number of reflections	6122	561
<I/σ(I)>	11.4
Completeness [%]	98.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289.15	0.1M Bis-Tris, 28% w/v Polyethylene glycol monomethyl ether 2000