7F1Y
L-lactate oxidase without substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 |
| Unit cell lengths | 132.298, 132.298, 91.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.660 - 1.330 |
| R-factor | 0.17142 |
| Rwork | 0.170 |
| R-free | 0.19261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 167253 | 16501 |
| <I/σ(I)> | 15.76 | |
| Completeness [%] | 98.4 | |
| Redundancy | 20 | |
| CC(1/2) | 0.984 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 20 mg/ml in 50 mM sodium acetate (pH4.5) , 40% ethylene glycol |
