7F1X

X-ray crystal structure of visual arrestin complexed with inositol 1,4,5-triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-28
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	167.566, 187.702, 190.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.925 - 3.000
R-factor	0.2077
Rwork	0.206
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cf1
RMSD bond length	0.003
RMSD bond angle	0.560
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.075	0.469
Rmeas		0.504
Rpim	0.030	0.183
Number of reflections	59857	8712
<I/σ(I)>	19.8
Completeness [%]	99.2
Redundancy	7.3
CC(1/2)	0.998	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000