7F0Z
Crystal structure of NsrQ W31A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.769, 52.150, 61.525 |
| Unit cell angles | 90.00, 94.81, 90.00 |
Refinement procedure
| Resolution | 45.610 - 2.000 |
| R-factor | 0.2086 |
| Rwork | 0.203 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f0o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.975 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.610 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.650 |
| Number of reflections | 19630 | 5702 |
| <I/σ(I)> | 6.3 | 1.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.997 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM citrate buffer (pH 5.5), 980 mM (NH4)2SO4 |
