7F0U
porcine epidemic diarrhea virus papain-like protease 2 C44S mutant in complex with mono ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.130, 86.451, 90.696 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 29.180 - 2.200 |
| R-factor | 0.2271 |
| Rwork | 0.226 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6noz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.355 |
| Rmeas | 0.058 | 0.416 |
| Rpim | 0.030 | 0.215 |
| Number of reflections | 29253 | 2883 |
| <I/σ(I)> | 25.1 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 19% PEG-3350, 50 mM HEPES pH 8.0 and 1% w/v tryptone |
