7F09

Crystal structure of the HLH-Lz domain of human TFE3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	27.940, 47.370, 69.960
Unit cell angles	85.24, 87.21, 88.32

Refinement procedure

Resolution	18.833 - 2.600
R-factor	0.2219
Rwork	0.221
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6KU4
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.190	47.190	2.720
High resolution limit [Å]	2.600	9.010	2.600
Rmerge	0.041	0.027	0.253
Rmeas	0.058	0.038	0.357
Rpim	0.041	0.027	0.252
Total number of observations		365	2316
Number of reflections	17459	218	1299
<I/σ(I)>	10.3	19.8	3.2
Completeness [%]	94.6	83.8	97.1
Redundancy	1.8	1.7	1.8
CC(1/2)	0.998	0.997	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Zinc Acetate, Zinc Chloride, Bis-Tris pH 7.5, PEG 3350, PEG 2000, PEG 4000 and PEG 5000MME