7F00
Crystal structure of SPD_0310
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.854, 87.854, 326.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.700 |
| R-factor | 0.2231 |
| Rwork | 0.221 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bh1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.045 | 0.552 |
| Number of reflections | 36236 | 3027 |
| <I/σ(I)> | 30.9 | 2.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 290 | 2.0 M Ammonium Sulfate and 0.1 M Hepes, pH 7.5 |
