7EZI
Rice L-galactose dehydrogenase (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-01-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.745, 54.877, 56.753 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 32.092 - 1.200 |
| Rwork | 0.152 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lut |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.077 | 0.611 |
| Number of reflections | 87015 | 3919 |
| <I/σ(I)> | 17.4 | 4.1 |
| Completeness [%] | 99.1 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M bis-tris pH 6.5, 0.2M MgCl2, 25% poly- ethylene glycol 3350 |
