7EYU
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-27 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.813, 50.583, 71.982 |
| Unit cell angles | 109.11, 91.29, 112.86 |
Refinement procedure
| Resolution | 27.850 - 2.050 |
| R-factor | 0.2433 |
| Rwork | 0.241 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eyr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.633 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.176 | 0.455 |
| Number of reflections | 31258 | 2440 |
| <I/σ(I)> | 7.1 | 3.5 |
| Completeness [%] | 96.2 | 95.7 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 0.971 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2 M magnesium formate dehydrate, 20% w/v PEG 3350 |
