7EYL

Crystal structure of Salmonella enterica ppnP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 31
Unit cell lengths	34.571, 34.571, 124.885
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.200
R-factor	0.1746
Rwork	0.174
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eyj
RMSD bond length	0.004
RMSD bond angle	0.841
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.070	0.198
Rmeas	0.079	0.227
Rpim	0.037	0.110
Number of reflections	51977	5098
<I/σ(I)>	7.824	7.824
Completeness [%]	99.7	98.8
Redundancy	4.3	4.2
CC(1/2)	0.994	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350