7EXZ

DgpB-DgpC complex apo 2.5 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	86.516, 156.721, 158.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.890 - 2.500
R-factor	0.1831
Rwork	0.182
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bvr
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.890	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.122	0.815
Number of reflections	75507	4426
<I/σ(I)>	12	2.1
Completeness [%]	100.0	100
Redundancy	6.9	5.9
CC(1/2)	0.997	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris (pH 8.5), 24% PEG4000