7EXW

GH127 beta-L-arabinofuranosidase HypBA1 covalently complexed with alpha-L-arabinofuranosylamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	74.757, 74.757, 252.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.200 - 2.200
R-factor	0.2197
Rwork	0.217
R-free	0.26950
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3wkx
RMSD bond length	0.007
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.154	0.782
Rpim	0.066	0.309
Number of reflections	42003	2055
<I/σ(I)>	13.2	1.4
Completeness [%]	98.7
Redundancy	6.1
CC(1/2)	0.975	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.7 M sodium citrate, 0.1 M MES-NaOH (pH 6.5)