7EXS
Thermomicrobium roseum sarcosine oxidase mutant - S320R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 291.15 |
| Detector technology | CCD |
| Collection date | 2021-01-08 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.362, 47.141, 64.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.180 - 1.420 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gf3 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.377 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.180 | 1.500 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.086 | 0.760 |
| Number of reflections | 76088 | 10967 |
| <I/σ(I)> | 14.4 | 2.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | Bis, tris, ammonium sulfate |
