7EXK

An AA9 LPMO of Ceriporiopsis subvermispora

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	RAYONIX MX225HE
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	84.063, 52.428, 134.625
Unit cell angles	90.00, 90.55, 90.00

Refinement procedure

Resolution	48.850 - 2.140
R-factor	0.1752
Rwork	0.172
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b5q
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.270
High resolution limit [Å]	2.140	2.140
Rmerge	0.065	0.242
Rmeas	0.073	0.284
Number of reflections	61984	7982
<I/σ(I)>	15.46	4.49
Completeness [%]	95.5	76.5
Redundancy	4.9	3.56
CC(1/2)	0.998	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	293	0.78 mg/mL protein with equal volume of 0.1 M CaCl2, 20%(w/v) PEG3350