7EXK
An AA9 LPMO of Ceriporiopsis subvermispora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.063, 52.428, 134.625 |
| Unit cell angles | 90.00, 90.55, 90.00 |
Refinement procedure
| Resolution | 48.850 - 2.140 |
| R-factor | 0.1752 |
| Rwork | 0.172 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b5q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.065 | 0.242 |
| Rmeas | 0.073 | 0.284 |
| Number of reflections | 61984 | 7982 |
| <I/σ(I)> | 15.46 | 4.49 |
| Completeness [%] | 95.5 | 76.5 |
| Redundancy | 4.9 | 3.56 |
| CC(1/2) | 0.998 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | 0.78 mg/mL protein with equal volume of 0.1 M CaCl2, 20%(w/v) PEG3350 |
