7EX4

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.933, 70.566, 70.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.410 - 2.000
R-factor	0.2067
Rwork	0.205
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.105	0.078	0.344
Rmeas	0.127	0.097	0.403
Rpim	0.071	0.056	0.206
Total number of observations	39371
Number of reflections	12910	1329	1162
<I/σ(I)>	10
Completeness [%]	88.5	84	84.1
Redundancy	3	2.8	3.4
CC(1/2)		0.993	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.1	289	PEG 3350