7EX1
Crystal structure of Ebinur Lake virus cap snatching endonuclease (Y109A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.528, 40.812, 74.577 |
Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
Resolution | 46.500 - 2.300 |
R-factor | 0.2219 |
Rwork | 0.220 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xi5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.091 | 0.069 | 0.483 |
Rmeas | 0.106 | 0.081 | 0.557 |
Rpim | 0.052 | 0.041 | 0.270 |
Total number of observations | 72606 | ||
Number of reflections | 18574 | 1808 | 1870 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 97.0 | 90.4 | 98.6 |
Redundancy | 3.9 | 3.5 | 3.8 |
CC(1/2) | 0.994 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5 | 289 | PEG 3350 |